PHYSIOLOGICAL CHARACTERISTICS OF HUMAN RED BLOOD CELL GHOSTS
نویسندگان
چکیده
منابع مشابه
Physiological Characteristics of Human Red Blood Cell Ghosts
The properties of ghosts prepared by hypotonic hemolysis at various ratios of cells (C) to hemolyzing solution (H) have been studied. At all ratios, hemoglobin (Hb) was found to be distributed equally between the ghost and supernatant compartments. Techniques employing Fe(59)-labelled Hb showed that during hemolysis all of the Hb is exchangeable and that following hemolysis the ghost is imperme...
متن کاملPhysiological Characteristics of Human Red Blood
The properties of ghosts prepared by hypotonic hemolysis at various ratios of cells (C) to hemolyzing solution (H) have been studied. At all ratios, hemoglobin (I-l-b) was found to be distributed equally between the ghost and supernatant compartments. Techniques employing FeSg-labelled Hb showed that during hemolysis all of the Hb is exchangeable and that following hemolysis the ghost is imperm...
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The type of birefringence described by Mitchison, which extends some 0.5 micro in from the surface of the human red cell ghost in glycerol and which shows a maximum retardation of about 7 A, is only found in ghosts which are sufficiently well hemoglobinised to be seen with the ordinary microscope. Ghosts from which all hemoglobin has been lost are not visible with the ordinary microscope and ar...
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K influx into resealed human red cell ghosts increases when the ghosts are swollen. The influx demonstrates properties similar to volume-sensitive K fluxes present in other cells. The influx is, for the most part, insensitive to the nature of the major intracellular cation and therefore is not a K-K exchange. The influx is much greater when the major anion is Cl than when the major anion is NO3...
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The rate of Ca influx into ghosts containing arsenazo III changes with time, being most rapid during the first 5 min after Ca is added to the outside and declining thereafter. The rate of Ca influx is a nonlinear function of extracellular Ca and plateaus as the latter is increased above 1 mM. The rate of Ca influx was measured as a function of the transmembrane gradients of Na and K and changes...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1958
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.42.1.9